(Secondary Ownership. The experiment uses only third-party data.)
Abstract from Pubmed:
The master transcription factor, Ppar-gamma regulates the general differentiation program of both brown and white adipocytes. However, it has been unclear whether Ppar-gamma also controls fat lineage-specific characteristics. Here, we show that Early B-Cell Factor-2 (Ebf2) regulates Ppar-gamma binding activity to determine brown versus white adipocyte identity. The Ebf DNA-binding motif was highly enriched within brown adipose-specific Ppar-gamma binding sites that we identified by genomewide ChIP-Seq. Of the Ebf isoforms, Ebf2 was selectively expressed in brown relative to white adipocytes and was bound at brown adipose-specific Ppar-gamma target genes. When expressed in myoblasts or white pre-adipose cells, Ebf2 recruited Ppar-Gamma to its brown-selective binding sites and reprogrammed cells to a brown fat fate. Brown adipose cells and tissue from Ebf2-deficient mice displayed a loss of brown-specific characteristics and thermogenic capacity. Together, these results identify Ebf2 as a key transcriptional regulator of brown fat cell fate and function.
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